pb cre4 mice Search Results


probasin pb cre4 mice  (Jackson Laboratory)
86
Jackson Laboratory probasin pb cre4 mice
Probasin Pb Cre4 Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pten loxp loxp smad4 loxp loxp pb cre4  (Thermo Fisher)
99
Thermo Fisher pten loxp loxp smad4 loxp loxp pb cre4
a, b, Immunohistochemical (a) and western blot analysis (b) of wild-type (WT) and Ptenpc−/− use prostate tissues. Scale bar, 50 μm. c, Oncomine boxed plot of <t>SMAD4</t> expression levels between human PCA and metastasis in multiple data sets including those from ref. 19 and ref. 20. d, SMAD4 knockdown enhanced metastatic potential to lung from PC3 cells implanted in renal capsule of immunocompromised nude mice.
Pten Loxp Loxp Smad4 Loxp Loxp Pb Cre4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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il17rc / mice  (Genentech inc)
90
Genentech inc il17rc / mice
a, b, Immunohistochemical (a) and western blot analysis (b) of wild-type (WT) and Ptenpc−/− use prostate tissues. Scale bar, 50 μm. c, Oncomine boxed plot of <t>SMAD4</t> expression levels between human PCA and metastasis in multiple data sets including those from ref. 19 and ref. 20. d, SMAD4 knockdown enhanced metastatic potential to lung from PC3 cells implanted in renal capsule of immunocompromised nude mice.
Il17rc / Mice, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rb1 tm2brn j  (Jackson Laboratory)
86
Jackson Laboratory rb1 tm2brn j
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
Rb1 Tm2brn J, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c57bl6 background  (Jackson Laboratory)
86
Jackson Laboratory c57bl6 background
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
C57bl6 Background, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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collagenase solution  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc collagenase solution
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
Collagenase Solution, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b6 129s4 pten tm1hwu j mice  (Jackson Laboratory)
86
Jackson Laboratory b6 129s4 pten tm1hwu j mice
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
B6 129s4 Pten Tm1hwu J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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passive lysis buffer  (Promega)
90
Promega passive lysis buffer
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
Passive Lysis Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ptenloxp loxp  (Jackson Laboratory)
86
Jackson Laboratory ptenloxp loxp
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
Ptenloxp Loxp, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b6 129s4 ptentm1hwu j mice  (Jackson Laboratory)
86
Jackson Laboratory b6 129s4 ptentm1hwu j mice
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
B6 129s4 Ptentm1hwu J Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell strainers  (Becton Dickinson)
90
Becton Dickinson cell strainers
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
Cell Strainers, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p53flox mice  (Jackson Laboratory)
86
Jackson Laboratory p53flox mice
Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; <t>Rb1</t> f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.
P53flox Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a, b, Immunohistochemical (a) and western blot analysis (b) of wild-type (WT) and Ptenpc−/− use prostate tissues. Scale bar, 50 μm. c, Oncomine boxed plot of SMAD4 expression levels between human PCA and metastasis in multiple data sets including those from ref. 19 and ref. 20. d, SMAD4 knockdown enhanced metastatic potential to lung from PC3 cells implanted in renal capsule of immunocompromised nude mice.

Journal: Nature

Article Title: SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression

doi: 10.1038/nature09677

Figure Lengend Snippet: a, b, Immunohistochemical (a) and western blot analysis (b) of wild-type (WT) and Ptenpc−/− use prostate tissues. Scale bar, 50 μm. c, Oncomine boxed plot of SMAD4 expression levels between human PCA and metastasis in multiple data sets including those from ref. 19 and ref. 20. d, SMAD4 knockdown enhanced metastatic potential to lung from PC3 cells implanted in renal capsule of immunocompromised nude mice.

Article Snippet: Establishment of mouse prostate tumour cell lines Tumours were dissected from prostates of Pten loxp/loxp Smad4 loxp/loxp PB-Cre4 + ( Pten pc −/− Smad4 pc −/− ) mice, minced, and digested with 0.5% type I collagenase (Invitrogen) as described previously.

Techniques: Immunohistochemical staining, Western Blot, Expressing, Knockdown

a, The four-gene set of PTEN/SMAD4/CCND1/SPP1 can dichotomize PCA cases for BCR in the ref. 13 data set. b, c, C-statistic analysis revealed that this four-gene set can enhance the prognostic accuracy of Gleason score in the ref. 13 data set (b) and in an independent PHS cohort (c). d, TMA-based four-protein model also significantly improve the prognostic ability of Gleason (P = 0.015) from the PHS cohort. e, Representative immunohistochemical staining with specific antibody against PTEN, SMAD4, CCND1 and SPP1 in the Directors Challenge TMA. Scale bar, 200 μm.

Journal: Nature

Article Title: SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression

doi: 10.1038/nature09677

Figure Lengend Snippet: a, The four-gene set of PTEN/SMAD4/CCND1/SPP1 can dichotomize PCA cases for BCR in the ref. 13 data set. b, c, C-statistic analysis revealed that this four-gene set can enhance the prognostic accuracy of Gleason score in the ref. 13 data set (b) and in an independent PHS cohort (c). d, TMA-based four-protein model also significantly improve the prognostic ability of Gleason (P = 0.015) from the PHS cohort. e, Representative immunohistochemical staining with specific antibody against PTEN, SMAD4, CCND1 and SPP1 in the Directors Challenge TMA. Scale bar, 200 μm.

Article Snippet: Establishment of mouse prostate tumour cell lines Tumours were dissected from prostates of Pten loxp/loxp Smad4 loxp/loxp PB-Cre4 + ( Pten pc −/− Smad4 pc −/− ) mice, minced, and digested with 0.5% type I collagenase (Invitrogen) as described previously.

Techniques: Immunohistochemical staining, Staining

Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; Rb1 f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.

Journal: Cancer Discovery

Article Title: Serotonin Modulates Lineage Plasticity in Neuroendocrine Prostate Cancer via Epigenetic Reprogramming

doi: 10.1158/2159-8290.CD-25-0974

Figure Lengend Snippet: Identification of serotonin signaling as a candidate target in NEPC through high-throughput compound screening and clinical transcriptomic data. A, A schematic showing the compound screening pipeline using the NEPC cell line LASCPC-01 and the CellTiter-Glo viability assay. B, A waterfall plot showing the relative viability of NEPC cells upon treatment with 1,112 FDA-approved compounds. C, Mechanism of action enrichment analysis from the Drug Repurposing Hub highlighting serotonin reuptake inhibitors as the top enriched class among active compounds. D, Serotonin-related targets identified from Drug Central among the top enriched hits. E, Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plot of scRNA-seq data from CRPC epithelial cells , showing NE cell clusters and SOX2 expression. F, A Venn diagram showing NE marker genes shared between human CRPC single-cell data and the TKO (Pb-Cre4: Pten f/f ; Trp53 f/f ; Rb1 f/f ) NEPC mouse model . Ranking of shared NE genes in the original Beltran and colleagues dataset . G, UMAP subclustering of epithelial cells showing SOX2 + DDC + , SOX2 + DDC − , and SOX2 − DDC − populations. H, A gene expression correlation heatmap between NE markers, AR pathway genes, and DDC / SLC6A4 using bulk RNA-seq data (Beltran and colleagues cohort; ref. ). I, Box plots showing expression levels of DDC and SLC6A4 in four public prostate cancer datasets (Beltran and colleagues, Tzelepi and colleagues, SU2C/Abida and colleagues, and Taylor and colleagues cohorts; refs. – ). J, Representative hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining of DDC, AR, and CHGA in prostate tissues from benign prostate hyperplasia (BPH, n = 10), hormone-sensitive prostate cancer (HSPC, n = 27), CRPC ( n = 13), and NEPC ( n = 15) patients. Scale bar, 100 μm. K, Representative H&E and IHC staining of DDC, AR, and CHGA in prostate, liver, and lung tumors from TKO and DKO (Pb-Cre4: Pten f/f ; Trp53 f/f ) mice. Scale bar, 100 μm.

Article Snippet: Tg (Pbsn-cre)4Prb/J (026662, RRID: IMSR_JAX:026662), B6.129P2- Trp53 tm1Brn /J (008462, RRID: IMSR_JAX:008462), Rb1 tm2Brn /J (026563, RRID: IMSR_JAX:026563), and B6.129S4- Pten tm1Hwu /J mice (006440, RRID: IMSR_JAX:006440) were bought from The Jackson Laboratory to generate TKO mice (Pb-Cre4: Pten f/f ; Trp53 f/f ; Rb1 f/f ), DKO mice (Pb-Cre4: Pten f/f ; Trp53 f/f ), and Pten f/f ; Trp53 f/f ; Rb1 f/f mice.

Techniques: High Throughput Screening Assay, Viability Assay, Expressing, Marker, Single Cell, Gene Expression, RNA Sequencing, Immunohistochemistry